Research
Expression and Clinicopathological Significance of Hedgehog Signaling Pathway in Malignant Mesothelioma
Guan-Ying Ma, Shuai Shi, Ping Wang, Zhi-Gang Zhang*
Department of Clinical Pathology, Chengde Medical University, Chengde 067000, China
*Corresponding author: Zhi-Gang Zhang, Department of Clinical Pathology, Chengde Medical University, Chengde 067000, China.
Received: October 14, 2021; Published: November 20, 2021
Research
Background: To investigate the expression of Hedgehog signalling pathway gene in malignant
mesothelioma and benign mesothelioma reactive hyperplasia by immunohistochemistry, and to compare the diagnostic value of pleural and abdominal fluid samples in malignant mesothelioma.
Methods: In this study, 70 cases of chest and abdominal effusion specimens confirmed by surgical endoscopic biopsy in our hospital from January 2016 to December 2019 were selected, including 50 cases in the malignant mesothelioma group and 20 cases in the benign reactive mesothelioma group. Biopsy and effusion samples were examined by immunohistochemistry at the same time, and the protein expression of Smo and GLi1 genes in Hedgehog signalling pathway was compared to further analyze the application value in the diagnosis of thoracic and abdominal effusion samples of malignant mesothelioma.
Results: The positive rates of Smo and GLi1 in biopsy specimens were 94.00% and 90.00%, respectively. Their specificity was 85.71% and 77.27%, and their sensitivity was 95.92% and 93.75%. The positive rate of Smo was 90.00% and 88.00%, the specificity was 77.27% and 71.43%, and the sensitivity was 93.75% and 89.80%, respectively. It proved that the diagnostic results of chest and ascites specimens were consistent with those of biopsy specimens. After statistical analysis of clinic-pathological data, the prognosis of malignant mesothelioma was positively correlated with asbestos chemical exposure history (P<0.05), but had little correlation with sex, age, lesion site, proliferation index gene P53 expression Figure 1-12 and Table 1-4.
Conclusion: Therefore, the gene detection of thorax and ascites in Hedgehog signalling pathway can be used as a new method for diagnosis of malignant mesothelioma.
Keywords: Malignant Mesothelioma; Hedgehog Signalling Pathway; Protein Expression; Thoracoabdominal Effusion; Immunohistochemistry
Citation: Zhi-Gang Zhang., et al., (2021) “Expression and Clinicopathological Significance of Hedgehog Signaling Pathway in Malignant Mesothelioma.” Sis Med Dent Archives J 1(1): 23-34.
Copyright: 2021 Zhi-Gang Zhang., et al., This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.